Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Lect2 deficiency is characterised by altered cytokine levels and promotion of intestinal tumourigenesis

Greenow, Kristy R., Zverev, Matthew, May, Stephanie, Kendrick, Howard, Williams, Geraint T. ORCID: https://orcid.org/0000-0003-3768-9940, Phesse, Toby ORCID: https://orcid.org/0000-0001-9568-4916 and Parry, Lee ORCID: https://orcid.org/0000-0002-4467-9196 2018. Lect2 deficiency is characterised by altered cytokine levels and promotion of intestinal tumourigenesis. Oncotarget 9 (92) , pp. 36430-36443. 10.18632/oncotarget.26335

[thumbnail of 26335-1015630-6-PB.pdf]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (4MB) | Preview

Abstract

Leukocyte cell-derived chemotaxin 2 (Lect2) is a chemokine-like chemotactic factor that has been identified as a downstream target of the Wnt signalling pathway. Whilst the primary function of Lect2 is thought to be in modulating the inflammatory process, it has recently been implicated as a potential inhibitor of the Wnt pathway. Deregulation of the Wnt pathway, often due to loss of the negative regulator APC, is found in ~80% of colorectal cancer (CRC). Here we have used the ApcMin/+Lect2−/− mouse model to characterise the role of Lect2 in Wnt-driven intestinal tumourigenesis. Histopathological, immunohistochemical, PCR and flow cytometry analysis were employed to identify the role of Lect2 in the intestine. The ApcMin/+Lect2−/− mice had a reduced mean survival and a significantly increased number of adenomas in the small intestine with increased severity. Analysis of Lect2 loss indicated it had no effect on the Wnt pathway in the intestine but significant differences were observed in circulating inflammatory markers, CD4+ T cells, and T cell lineage-specification factors. In summary, in the murine intestine loss of Lect2 promotes the initiation and progression of Wnt-driven colorectal cancer. This protection is performed independently of the Wnt signalling pathway and is associated with an altered inflammatory environment during Wnt-driven tumorigenesis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Medicine
European Cancer Stem Cell Research Institute (ECSCRI)
Additional Information: This is an open access article under the terms of the CC-BY license.
Publisher: Impact Journals
ISSN: 1949-2553
Date of First Compliant Deposit: 1 November 2018
Date of Acceptance: 28 October 2018
Last Modified: 23 Oct 2023 06:25
URI: https://orca.cardiff.ac.uk/id/eprint/116399

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics