Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression

Gurevich, David B., Severn, Charlotte E., Twomey, Catherine, Greenhough, Alexander, Cash, Jenna, Toye, Ashley M., Mellor, Harry and Martin, Paul ORCID: https://orcid.org/0000-0002-2665-5086 2018. Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression. EMBO Journal 37 (13) , e97786. 10.15252/embj.201797786

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Abstract

Wound angiogenesis is an integral part of tissue repair and is impaired in many pathologies of healing. Here, we investigate the cellular interactions between innate immune cells and endothelial cells at wounds that drive neoangiogenic sprouting in real time and in vivo. Our studies in mouse and zebrafish wounds indicate that macrophages are drawn to wound blood vessels soon after injury and are intimately associated throughout the repair process and that macrophage ablation results in impaired neoangiogenesis. Macrophages also positively influence wound angiogenesis by driving resolution of anti‐angiogenic wound neutrophils. Experimental manipulation of the wound environment to specifically alter macrophage activation state dramatically influences subsequent blood vessel sprouting, with premature dampening of tumour necrosis factor‐α expression leading to impaired neoangiogenesis. Complementary human tissue culture studies indicate that inflammatory macrophages associate with endothelial cells and are sufficient to drive vessel sprouting via vascular endothelial growth factor signalling. Subsequently, macrophages also play a role in blood vessel regression during the resolution phase of wound repair, and their absence, or shifted activation state, impairs appropriate vessel clearance.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Additional Information:	This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Publisher:	EMBO Press
ISSN:	0261-4189
Date of First Compliant Deposit:	10 May 2019
Date of Acceptance:	30 April 2018
Last Modified:	03 May 2023 11:51
URI:	https://orca.cardiff.ac.uk/id/eprint/122331

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