The development of the respiratory chain of Saccharomyces carlsbergensis during respiratory adaptation.

Cartledge, T. G., Lloyd, D. ORCID: https://orcid.org/0000-0002-5656-0571, Erecińska, M. and Chance, B. 1972. The development of the respiratory chain of Saccharomyces carlsbergensis during respiratory adaptation. Biochemical Journal 130 (3) , pp. 739-747. 10.1042/bj1300739

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Abstract

Subcellular fractionation of sphaeroplasts produced at different stages during the first 4h of respiratory adaptation of anaerobically grown glucose-de-repressed Saccharomyces carlsbergensis gave mitochondrial fractions that contained all the detectable c- and a-type cytochromes. 2. The rates of cytochrome formation were studied; individual cytochromes were produced at different rates so as to give respiratory chains having widely differing cytochrome ratios. A CO-reacting haemoprotein other than cytochrome a(3) also increased throughout 8h of respiratory adaptation. 3. Even after short periods of aeration, organisms contained mitochondria in which cytochrome-cytochrome interactions and the reaction of cytochrome a(3) with O(2) proceeded at rates almost as fast as in organelles from aerobically grown cells. 4. The technique of flow-flash photolysis enabled kinetic resolution of the reoxidation of cytochromes a(3) and a to be achieved and their individual contributions to extinction changes in the Soret region were assessed. The ratio cytochrome a(3)/cytochrome a increased over the early stages of adaptation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Portland Press
ISSN:	0264-6021
Last Modified:	26 Oct 2022 08:37
URI:	https://orca.cardiff.ac.uk/id/eprint/127906

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