Quantitation of the growth and differentiation of normal and malignant bone marrow derived stromal cells

Almond, Wendy Rachel 2006. Quantitation of the growth and differentiation of normal and malignant bone marrow derived stromal cells. PhD Thesis, Cardiff University.

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Abstract

Normal and pathological passaged BMdSCs were successfully grown to confluence over 3 passages and induced to differentiate down the osteogenic and adipogenic lineage with the formation of mineral deposit and multidroplet cell clusters respectively. JMML BMdSCs had a significantly increased growth rate and MDS BMdSCs had a significantly increased number of small colonies in comparison to normal. Lymphoma BMdSCs had an increased CD105, CD49b and CD45 antigenic expression; myeloma BMdSCs had an increased CD105 antigenic expression, and the lymphoproliferative BMdSCs had a significantly reduced CD90, CD105 and CD49b expression in comparison to normal. With osteogenic induction, AML and lymphoma (mineralised) cultures had a reduced osteocalcin gene expression in comparison to normal. With adipogenic induction, AML and lymphoma cultures showed reduced multidroplet cell cluster formation with comparable gene expression to normal.

Item Type:	Thesis (PhD)
Status:	Unpublished
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics
Funders:	Medical Research Council
Date of First Compliant Deposit:	30 March 2016
Last Modified:	09 Jan 2018 18:55
URI:	https://orca.cardiff.ac.uk/id/eprint/54102

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