Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Rapid loss of intestinal crypts upon conditional deletion of the Wnt/Tcf-4 target gene c-Myc

Muncan, Vanesa, Sansom, Owen J. ORCID: https://orcid.org/0000-0001-9540-3010, Tertoolen, Leon, Phesse, Toby ORCID: https://orcid.org/0000-0001-9568-4916, Begthel, Harry, Sancho, Elena, Cole, Alicia M., Gregorieff, Alex, de Alboran, Ignacio Moreno, Clevers, Hans and Clarke, Alan Richard ORCID: https://orcid.org/0000-0002-4281-426X 2006. Rapid loss of intestinal crypts upon conditional deletion of the Wnt/Tcf-4 target gene c-Myc. Molecular and Cellular Biology 26 (22) , pp. 8418-8426. 10.1128/MCB.00821-06

[thumbnail of Erratum]
Preview
PDF (Erratum) - Updated Version
Download (242kB) | Preview

Abstract

Inhibition of the mutationally activated Wnt cascade in colorectal cancer cell lines induces a rapid G1 arrest and subsequent differentiation. This arrest can be overcome by maintaining expression of a single Tcf4 target gene, the proto-oncogene c-Myc. Since colorectal cancer cells share many molecular characteristics with proliferative crypt progenitors, we have assessed the physiological role of c-Myc in adult crypts by conditional gene deletion. c-Myc-deficient crypts are lost within weeks and replaced by c-Myc-proficient crypts through a fission process of crypts that have escaped gene deletion. Although c-Myc−/− crypt cells remain in the cell cycle, they are on average much smaller than wild-type cells, cycle slower, and divide at a smaller cell size. c-Myc appears essential for crypt progenitor cells to provide the necessary biosynthetic capacity to successfully progress through the cell cycle.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Subjects: Q Science > Q Science (General)
Publisher: American Society for Microbiology
ISSN: 0270-7306
Last Modified: 29 Jun 2023 17:06
URI: https://orca.cardiff.ac.uk/id/eprint/61347

Citation Data

Cited 176 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics