Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Crystal structures of VIM-1 complexes explain active site heterogeneity in VIM-class metallo-β-lactamases

Salimraj, Ramya, Hinchliffe, Philip, Kosmopoulou, Magda, Tyrrell, Jonathan M. ORCID: https://orcid.org/0000-0001-8565-2590, Brem, Jürgen, van Berkel, Sander S., Verma, Anil, Owens, Raymond J., McDonough, Michael A., Walsh, Timothy R. ORCID: https://orcid.org/0000-0003-4315-4096, Schofield, Christopher J. and Spencer, James 2019. Crystal structures of VIM-1 complexes explain active site heterogeneity in VIM-class metallo-β-lactamases. FEBS Journal 286 (1) , pp. 169-183. 10.1111/febs.14695

[thumbnail of Salimraj_et_al-2018-The_FEBS_Journal.pdf]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (2MB) | Preview

Abstract

Metallo‐β‐Lactamases (MBLs) protect bacteria from almost all β‐lactam antibiotics. Verona integron‐encoded MBL (VIM) enzymes are among the most clinically important MBLs, with VIM‐1 increasing in carbapenem‐resistant Enterobacteriaceae (Escherichia coli, Klebsiella pneumoniae) that are among the hardest bacterial pathogens to treat. VIM enzymes display sequence variation at residues (224 and 228) that in related MBLs are conserved and participate in substrate binding. How they accommodate this variability, while retaining catalytic efficiency against a broad substrate range, has remained unclear. Here, we present crystal structures of VIM‐1 and its complexes with a substrate‐mimicking thioenolate inhibitor, ML302F, that restores meropenem activity against a range of VIM‐1 producing clinical strains, and the hydrolysed product of the carbapenem meropenem. Comparison of these two structures identifies a water‐mediated hydrogen bond, between the carboxylate group of substrate/inhibitor and the backbone carbonyl of the active site zinc ligand Cys221, that is common to both complexes. Structural comparisons show that the responsible Cys221‐bound water is observed in all known VIM structures, participates in carboxylate binding with other inhibitor classes, and thus effectively replicates the role of the conserved Lys224 in analogous complexes with other MBLs. These results provide a mechanism for substrate binding that permits the variation at positions 224 and 228 that is a hallmark of VIM MBLs.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Wiley: FEBS Journal
ISSN: 1742-464X
Date of First Compliant Deposit: 27 November 2018
Date of Acceptance: 2 November 2018
Last Modified: 06 May 2023 01:46
URI: https://orca.cardiff.ac.uk/id/eprint/117087

Citation Data

Cited 22 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics