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Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

Sedgwick, Adam C., Gardiner, Jordan E., Kim, Gyoungmi, Yevglevskis, Maksims, Lloyd, Matthew D., Jenkins, A. Toby A., Bull, Steven D., Yoon, Juyoung and James, Tony D. 2018. Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols. Chemical Communications 54 (38) , pp. 4786-4789. 10.1039/C8CC01661E

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Abstract

Two ‘turn on’ TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear ‘turn on’ with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Publisher: Royal Society of Chemistry
ISSN: 1359-7345
Date of First Compliant Deposit: 13 July 2020
Date of Acceptance: 16 April 2018
Last Modified: 15 Jul 2021 01:11
URI: http://orca.cardiff.ac.uk/id/eprint/133374

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