A single-nucleus transcriptomic atlas of the adult Aedes aegypti mosquito

Goldman, Olivia V., DeFoe, Alexandra E., Qi, Yanyan, Jiao, Yaoyu, Weng, Shih-Che, Wick, Brittney, Houri-Zeevi, Leah, Lakhiani, Priyanka, Morita, Takeshi, Razzauti, Jacopo, Rosas-Villegas, Adriana, Tsitohay, Yael N., Walker, Madison M., Hopkins, Ben R., Haeussler, Maximilian, Akbari, Omar S., Duvall, Laura B., White-Cooper, Helen ORCID: https://orcid.org/0000-0002-3373-8023, Sorrells, Trevor R., Sharma, Roshan, Li, Hongjie, Vosshall, Leslie B. and Shai, Nadav 2025. A single-nucleus transcriptomic atlas of the adult Aedes aegypti mosquito. Cell 118 10.1016/j.cell.2025.10.008

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Abstract

The female Aedes aegypti mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Aedes aegypti Mosquito Cell Atlas, a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues of adult female and male Aedes aegypti, providing cellular-level resolution of mosquito biology. We identify novel cell types and expand our understanding of sensory neuron organization of chemoreceptors across all sensory tissues. Our analysis uncovers male-specific cells and sexually dimorphic gene expression in the antenna and brain. In female mosquitoes, we find that glial cells, rather than neurons, undergo the most extensive transcriptional changes in the brain following blood feeding. Our findings provide insights into the cellular basis of mosquito behavior and sexual dimorphism. The Aedes aegypti Mosquito Cell Atlas resource enables systematic investigation of cell-type-specific expression across all mosquito tissues. [Abstract copyright: Copyright © 2025 The Author(s). Published by Elsevier Inc. All rights reserved.]

Item Type:	Article
Date Type:	Publication
Status:	In Press
Schools:	Schools > Biosciences
Publisher:	Elsevier
ISSN:	0092-8674
Date of First Compliant Deposit:	20 November 2025
Date of Acceptance:	3 October 2025
Last Modified:	20 Nov 2025 14:00
URI:	https://orca.cardiff.ac.uk/id/eprint/182539

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