Bioluminescent Listeria monocytogenes provide a rapid assay for measuring biocide efficacy

Walker, Athene J., Jassim, Sabah A. A., Holah, John T., Denyer, Stephen Paul and Stewart, Gordon S. A. B. 1992. Bioluminescent Listeria monocytogenes provide a rapid assay for measuring biocide efficacy. FEMS Microbiology Letters 91 (3) , pp. 251-256. 10.1111/j.1574-6968.1992.tb05217.x

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Abstract

A recombinant derivative of Listeria monocytogenes 23074, engineered to express the luxAB genes of Vibrio fischeri MJ1, has a bioluminescent phenotype that provides a rapid monitor of microbial viability. The antibacterial activity of phenol and chlorhexidine diacetate (Hibitane) was measured using both bioluminescence and viable counts. Concentration exponents were assessed as 7.3 for phenol and 2.63 for chlorhexidine diacetate using plate counts. The rapidity of bioluminescence measurement constitutes a major advantage in biocide assessment.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	Q Science > QR Microbiology
Uncontrolled Keywords:	Bioluminescence; Listeria monocytogenes; Phenol; Chlorhexidine diacetate
Publisher:	Wiley-Blackwell
ISSN:	0378-1097
Last Modified:	19 Mar 2016 23:14
URI:	https://orca.cardiff.ac.uk/id/eprint/44248

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