Cell and protein adhesion studies in glaucoma drainage device development

Lim, K. S., Faragher, R. G., Reed, S., Wong, L., Olliff, C. J., Hanlon, G. W., Gard, P., Willis, S., Denyer, Stephen Paul, Muir, A., Lloyd, A. W., Khaw, P. T. and Allan, B. D. S. 1999. Cell and protein adhesion studies in glaucoma drainage device development. British Journal of Ophthalmology 83 (10) , pp. 1168-1171. 10.1136/bjo.83.10.1168

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Abstract

AIM: To examine in vitro whether phosphorylcholine coating of poly(methylmethacrylate) can reduce the adhesion of fibrinogen, fibrin, human scleral fibroblast and macrophage compared with current biomaterials used in the construction of glaucoma drainage devices. METHODS: Sample discs (n=6) of poly(methylmethacrylate), silicone, polypropylene, PTFE, and phosphorylcholine coated poly(methylmethacrylate) were seeded with fibrinogen, fibrin, fibroblast, and macrophages and incubated for variable lengths of time. The quantification was performed using radioactivity, spectrophotometry, ATP dependent luminometry, and immunohistochemistry respectively. RESULTS: Fibrinogen and fibrin adhesion to phosphorylcholine coated poly(methylmethacrylate) were significantly lower than PMMA (p=0.004). Phosphorylcholine coating of poly(methylmethacrylate) also significantly reduced the adhesion of human scleral fibroblast (p=0.002) and macrophage (p=0.01) compared with PMMA. All the other biomaterials showed either similar or insignificantly different levels of adhesion to all the proteins and cells tested compared with PMMA. CONCLUSION: Phosphorylcholine coating is a new material technology that offers considerable promise in the field of glaucoma drainage device development.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RE Ophthalmology R Medicine > RM Therapeutics. Pharmacology
Publisher:	BMJ Publishing Group
ISSN:	0007-1161
Last Modified:	19 Mar 2016 23:14
URI:	https://orca.cardiff.ac.uk/id/eprint/44285

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