Induction of interleukin-8 in lung epithelial cells by Gram negative bacteria and its modulation by pulmonary surfactant lipids.

Alghaithy, Abdulaziz 2006. Induction of interleukin-8 in lung epithelial cells by Gram negative bacteria and its modulation by pulmonary surfactant lipids. PhD Thesis, Cardiff University.

PDF - Accepted Post-Print Version Download (53MB)

Abstract

The surfactants did not inhibit IL-8 induced by IL-1[Special character omitted.] in A549 cells suggesting that they act at the level of the LPS receptor complex. Disruption of membrane micro-domains ('rafts') with methyl-[Special character omitted.]-cyclodextrin significantly inhibited bacteria and LPS induced IL-8 in A549 cells. Isolation of membrane raft containing fractions by sucrose density gradient ultra-centrifugation showed that TLR4 is recruited into membrane lipid rafts on cell stimulation with the bacteria or LPS. The surfactants inhibited the bacterial and LPS mediated translocation of TLR4 into raft domains suggesting that their mechanism of action involves inhibition of LPS receptor complex formation in lipid raft domains.

Item Type:	Thesis (PhD)
Status:	Unpublished
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Date of First Compliant Deposit:	30 March 2016
Last Modified:	10 Oct 2017 15:22
URI:	https://orca.cardiff.ac.uk/id/eprint/54104

Actions (repository staff only)

Edit Item