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Microsatellites for the net-spinning caddisfly Plectrocnemia conspersa (Polycentropodidae)

Wilcock, Helen R., Hildrew, A., Nichols, R. A. and Bruford, Michael William 2001. Microsatellites for the net-spinning caddisfly Plectrocnemia conspersa (Polycentropodidae). Molecular Ecology Notes 1 (4) , pp. 318-319. 10.1046/j.1471-8278.2001.00123.x

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Six polymorphic microsatellites were developed for the caddisfly Plectrocnemia conspersa from two enriched partial genomic libraries. These represent the first microsatellites published for the order Trichoptera. We show that, whilst it is possible to develop a highly enriched library, isolation of polymorphic microsatellites is difficult for this species, as has been found in some other of invertebrate groups. The genotypes of 160 individuals were determined using these new loci. Observed heterozygosities ranged from 0.013 to 0.788. Despite their problematic isolation, polymorphic microsatellite loci show greater levels of variation than previously studied allozyme markers.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Sustainable Places Research Institute (PLACES)
Uncontrolled Keywords: caddisfly; freshwater insect; microsatellite DNA; Trichoptera
Publisher: Wiley-Blackwell
ISSN: 1471-8278
Last Modified: 04 Jun 2017 06:43

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