Dijkman, Patricia M., Castell, Oliver K. ORCID: https://orcid.org/0000-0002-6059-8062, Goddard, Alan D., Munoz-Garcia, Juan C., de Graaf, Chris, Wallace, Mark I. and Watts, Anthony 2018. Dynamic tuneable G protein-coupled receptor monomer-dimer populations. Nature Communications 9 (1) , 1710. 10.1038/s41467-018-03727-6 |
Preview |
PDF
- Published Version
Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
G protein-coupled receptors (GPCRs) are the largest class of membrane receptors, playing a key role in the regulation of processes as varied as neurotransmission and immune response. Evidence for GPCR oligomerisation has been accumulating that challenges the idea that GPCRs function solely as monomeric receptors; however, GPCR oligomerisation remains controversial primarily due to the difficulties in comparing evidence from very different types of structural and dynamic data. Using a combination of single-molecule and ensemble FRET, double electron–electron resonance spectroscopy, and simulations, we show that dimerisation of the GPCR neurotensin receptor 1 is regulated by receptor density and is dynamically tuneable over the physiological range. We propose a “rolling dimer” interface model in which multiple dimer conformations co-exist and interconvert. These findings unite previous seemingly conflicting observations, provide a compelling mechanism for regulating receptor signalling, and act as a guide for future physiological studies.
Item Type: | Article |
---|---|
Date Type: | Publication |
Status: | Published |
Schools: | Pharmacy |
Additional Information: | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license |
Publisher: | Nature Research |
ISSN: | 2041-1723 |
Date of First Compliant Deposit: | 3 May 2018 |
Date of Acceptance: | 6 March 2018 |
Last Modified: | 02 May 2023 12:06 |
URI: | https://orca.cardiff.ac.uk/id/eprint/111201 |
Citation Data
Cited 87 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
Edit Item |