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Lyvianne Decourtye, Erik Mire, Maud Clemessy, Victor Heurtier, Tatiana Ledent, Iain C. Robinson, Patrice Mollard, Jacques Epelbaum, Michael J. Meaney, Sonia Garel, Yves Le Bouc, Laurent Kappeler
IGF-1 Induces GHRH Neuronal Axon Elongation during Early Postnatal Life in Mice
www.plosone.org
www.plosone.org
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514.6583 159.9307 m
524.1827 159.9307 l
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f*
537.6189 146.948 m
566.7591 146.948 l
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99.4235 370.3912 l
99.4235 381.8911 l
f*
99.9235 369.8912 m
36 369.8912 l
36 370.3912 l
99.4235 370.3912 l
f*
282.255 381.8911 m
282.255 369.8912 l
281.755 370.3912 l
281.755 381.8911 l
f*
282.255 369.8912 m
99.9235 369.8912 l
99.9235 370.3912 l
281.755 370.3912 l
f*
435.8339 381.8911 m
435.8339 369.8912 l
435.3338 370.3912 l
435.3338 381.8911 l
f*
435.8339 369.8912 m
282.255 369.8912 l
282.255 370.3912 l
435.3338 370.3912 l
f*
532.5523 381.8911 m
532.5523 369.8912 l
532.0522 370.3912 l
532.0522 381.8911 l
f*
532.5523 369.8912 m
435.8339 369.8912 l
435.8339 370.3912 l
532.0522 370.3912 l
f*
576 369.8912 m
532.5523 369.8912 l
532.5523 370.3912 l
576 370.3912 l
f*
99.9235 369.8912 m
99.9235 357.8913 l
99.4235 358.3913 l
99.4235 369.8912 l
f*
99.9235 357.8913 m
36 357.8913 l
36 358.3913 l
99.4235 358.3913 l
f*
282.255 369.8912 m
282.255 357.8913 l
281.755 358.3913 l
281.755 369.8912 l
f*
282.255 357.8913 m
99.9235 357.8913 l
99.9235 358.3913 l
281.755 358.3913 l
f*
435.8339 369.8912 m
435.8339 357.8913 l
435.3338 358.3913 l
435.3338 369.8912 l
f*
435.8339 357.8913 m
282.255 357.8913 l
282.255 358.3913 l
435.3338 358.3913 l
f*
532.5523 369.8912 m
532.5523 357.8913 l
532.0522 358.3913 l
532.0522 369.8912 l
f*
532.5523 357.8913 m
435.8339 357.8913 l
435.8339 358.3913 l
532.0522 358.3913 l
f*
576 357.8913 m
532.5523 357.8913 l
532.5523 358.3913 l
576 358.3913 l
f*
99.9235 357.8913 m
99.9235 345.8914 l
99.4235 346.3914 l
99.4235 357.8913 l
f*
99.9235 345.8914 m
36 345.8914 l
36 346.3914 l
99.4235 346.3914 l
f*
282.255 357.8913 m
282.255 345.8914 l
281.755 346.3914 l
281.755 357.8913 l
f*
282.255 345.8914 m
99.9235 345.8914 l
99.9235 346.3914 l
281.755 346.3914 l
f*
435.8339 357.8913 m
435.8339 345.8914 l
435.3338 346.3914 l
435.3338 357.8913 l
f*
435.8339 345.8914 m
282.255 345.8914 l
282.255 346.3914 l
435.3338 346.3914 l
f*
532.5523 357.8913 m
532.5523 345.8914 l
532.0522 346.3914 l
532.0522 357.8913 l
f*
532.5523 345.8914 m
435.8339 345.8914 l
435.8339 346.3914 l
532.0522 346.3914 l
f*
576 345.8914 m
532.5523 345.8914 l
532.5523 346.3914 l
576 346.3914 l
f*
576 312.6413 m
36 312.6413 l
36 313.1413 l
576 313.1413 l
f*
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BT
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1 TL
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(1.)Tj
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(and)Tj
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(antibodies.)Tj
ET
Q
q
1 j
1 J
0 w
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BT
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1 TL
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(Target)Tj
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(of)Tj
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(Catalog)Tj
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(or)Tj
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(of)Tj
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(Species)Tj
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(Raised)Tj
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(in)Tj
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(or)Tj
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(Agouti-Related)Tj
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(\(AGRP\))Tj
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(\(82131\))Tj
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(\(Mouse\))Tj
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(H-003-57)Tj
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(Rabbit,)Tj
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(1/)Tj
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(500)Tj
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(Akt)Tj
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(\(pan\))Tj
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(\(C67E7\))Tj
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(Rabbit)Tj
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(mAb)Tj
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(\(HRP)Tj
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(Conjugate\))Tj
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(Cell)Tj
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(Signaling)Tj
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(Technology,)Tj
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(#8596)Tj
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(Rabbit,)Tj
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(monoclonal)Tj
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(1/)Tj
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(2000)Tj
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(Chicken)Tj
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(IgY)Tj
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(Goat)Tj
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(Anti-Chicken)Tj
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(IgY)Tj
1.7311 0 Td
(H&L)Tj
2.1694 0 Td
(\(DyLight)Tj
3.9459 0 Td
(594\))Tj
11.5882 0 Td
(Abcam,)Tj
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(ab96949)Tj
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(Goat,)Tj
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(polyclonal)Tj
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(1/)Tj
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(400)Tj
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(Digoxigenin)Tj
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(Anti-Digoxigenin-AP,)Tj
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(Fab)Tj
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(fragments)Tj
13.8256 0 Td
(ROCHE,)Tj
4.1198 0 Td
(11093274910)Tj
13.7652 0 Td
(Sheep,)Tj
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(polyclonal)Tj
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(1/)Tj
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(2000)Tj
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(Erk1/2)Tj
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(p44/42)Tj
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(MAPK)Tj
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(\(Erk1/2\))Tj
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(\(137F5\))Tj
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(Rabbi)Tj
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(mAb)Tj
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(Cell)Tj
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(Signaling)Tj
4.3163 0 Td
(Technology,)Tj
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(#4695)Tj
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(Rabbit,)Tj
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(monoclonal)Tj
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(1/)Tj
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(1000)Tj
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(GFP)Tj
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(Anti-GFP)Tj
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(antibody)Tj
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(Abcam,)Tj
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(ab6556)Tj
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(Rabbit,)Tj
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(polyclonal)Tj
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(1/1000)Tj
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(GH)Tj
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(Rabbit)Tj
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(antiserum)Tj
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(to)Tj
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(rat)Tj
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(growth)Tj
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(hormone)Tj
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(for)Tj
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(National)Tj
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(of)Tj
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(diabetes)Tj
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(&)Tj
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(digestive)Tj
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(&)Tj
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(kidney)Tj
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(\(NIDDK\),)Tj
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(AFP5641801)Tj
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(1/20000)Tj
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(Anti-VC-15)Tj
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(Proteogenix,)Tj
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(home)Tj
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(made)Tj
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(Rabbit,)Tj
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(polyclonal)Tj
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(1/1000)Tj
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(IGF-1R)Tj
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(IGF-1)Tj
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(Receptor)Tj
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[()]TJ
/F0 1 Tf
0.8012 0 Td
(Antibody)Tj
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(Cell)Tj
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(Signaling)Tj
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(Technology,)Tj
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(#3027)Tj
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(Rabbit,)Tj
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(polyclonal)Tj
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(1/)Tj
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(1000)Tj
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(MECA-32)Tj
7.3777 0 Td
(Puried)Tj
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(Rat)Tj
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(Anti-Mouse)Tj
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(Panendothelial)Tj
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(Cell)Tj
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(Pharmingen,)Tj
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(553849)Tj
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(Rat,)Tj
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(monoclonal)Tj
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(1/500)Tj
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(Mek1)Tj
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(Anti-)Tj
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(Dual)Tj
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(specicity)Tj
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(mitogen-activated)Tj
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(MAP2K1)Tj
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(Biological)Tj
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(Rabbit,)Tj
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(polyclonal)Tj
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(1/)Tj
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(3000)Tj
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(Neurolament)Tj
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(Anti-160)Tj
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(Medium)Tj
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(Abcam,)Tj
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(ab72998)Tj
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(Chicken,)Tj
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(polyclonal)Tj
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(1/1000)Tj
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(Phospho)Tj
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(Akt)Tj
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(Phospho-Akt)Tj
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(\(Ser473\))Tj
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(\(D9E\))Tj
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(XP)Tj
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(Rabbit)Tj
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(mAb)Tj
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(Cell)Tj
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(Signaling)Tj
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(Technology,)Tj
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(#4060)Tj
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(Rabbit,)Tj
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(monoclonal)Tj
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(1/)Tj
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(2000)Tj
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(Phospho)Tj
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(Erk1/2)Tj
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(Phospho-p44/42)Tj
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(MAPK)Tj
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(\(Erk1/2\))Tj
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(\(Thr202/Tyr204\))Tj
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(\(D13.14.4E\))Tj
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(XP)Tj
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(Rabbit)Tj
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(mAb)Tj
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(Cell)Tj
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(Signaling)Tj
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(Technology,)Tj
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(#4370)Tj
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(Rabbit,)Tj
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(monoclonal)Tj
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(1/)Tj
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(2000)Tj
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(IGF-1R)Tj
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(Phospho-IGF-I)Tj
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[()]TJ
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(\(Tyr1135/1136\)/Insulin)Tj
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(Receptor)Tj
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4.2784 0 Td
[()]TJ
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0.8013 0 Td
(\(Tyr1150/1151\))Tj
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(\(19H7\))Tj
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(Rabbit)Tj
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(mAb)Tj
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(Cell)Tj
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(Signaling)Tj
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(Technology,)Tj
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(#3024)Tj
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(Rabbit,)Tj
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(monoclonal)Tj
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(1/)Tj
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(500)Tj
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(Phospho)Tj
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(Mek1)Tj
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(Anti-Mek1)Tj
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(\(phospho)Tj
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(S298\))Tj
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(antibody)Tj
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([EPR3338])Tj
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(Abcam,)Tj
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(ab96379)Tj
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(Rabbit,)Tj
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(monoclonal)Tj
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(1/)Tj
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(3000)Tj
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(PRL)Tj
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(Rabbit)Tj
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(antiserum)Tj
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(to)Tj
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(rat)Tj
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(Prolactin)Tj
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(for)Tj
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(immunocytochemistry)Tj
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(National)Tj
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(institute)Tj
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(of)Tj
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(diabetes)Tj
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(&)Tj
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(digestive)Tj
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(&)Tj
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(kidney)Tj
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(diseases)Tj
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(\(NIDDK\),)Tj
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(AFP5641801)Tj
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(Rabbit,)Tj
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(polyclonal)Tj
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(1/40000)Tj
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(Rabbit)Tj
3.1144 0 Td
(IgG)Tj
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(Biotinylated)Tj
5.3822 0 Td
(anti-rabbit)Tj
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(IgG)Tj
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(\(H+L\))Tj
11.1875 0 Td
(Vector)Tj
3.1144 0 Td
(Laboratories,)Tj
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(BA-1000)Tj
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(Goat,)Tj
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(polyclonal)Tj
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(1/200)Tj
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(Rabbit)Tj
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(IgG)Tj
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(Donkey)Tj
3.6057 0 Td
(Anti-Rabbit)Tj
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(IgG)Tj
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(H&L)Tj
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(\(DyLight)Tj
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(550\))Tj
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(preadsorbed)Tj
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(Abcam,)Tj
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(ab98499)Tj
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(Donkey,)Tj
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(polyclonal)Tj
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(1/)Tj
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(400)Tj
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(Rabbit)Tj
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(IgG)Tj
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(Goat)Tj
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(Anti-Rabbit)Tj
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(IgG)Tj
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(H&L)Tj
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(\(DyLight)Tj
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(488\))Tj
11.8906 0 Td
(Abcam,)Tj
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(ab96883)Tj
13.2663 0 Td
(Goat,)Tj
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(polyclonal)Tj
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(1/)Tj
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(400)Tj
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(Rabbit)Tj
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(IgG)Tj
5.9641 0 Td
(Anti-Rabbit)Tj
5.1629 0 Td
(IgG)Tj
1.8445 0 Td
(\(whole)Tj
3.1597 0 Td
(molecule\)-Peroxidase)Tj
17.3331 0 Td
(Sigma-Aldrich,)Tj
6.7201 0 Td
(A0545)Tj
11.2178 0 Td
(Goat,)Tj
2.6759 0 Td
(polyclonal)Tj
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(1/)Tj
1.0582 0 Td
(20000)Tj
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(Rat)Tj
1.7839 0 Td
(IgG)Tj
9.9932 0 Td
(Biotinylated)Tj
5.3746 0 Td
(anti-rat)Tj
3.3411 0 Td
(IgG)Tj
1.8445 0 Td
(\(H+L\))Tj
11.8527 0 Td
(Vector)Tj
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(Laboratories,)Tj
6.0473 0 Td
(BA-4001)Tj
10.1444 0 Td
(Rabbit,)Tj
3.3865 0 Td
(polyclonal)Tj
8.6477 0 Td
(1/200)Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 36 330.1228 cm
BT
/F0 1 Tf
1 TL
0 0 Td
(List)Tj
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(of)Tj
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(primaries)Tj
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(and)Tj
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(secondarie)Tj
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(s)Tj
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(antibodies)Tj
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(used)Tj
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(for)Tj
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(immunohis)Tj
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(tochemistry)Tj
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(and)Tj
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(western)Tj
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(blot)Tj
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(experimen)Tj
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(ts)Tj
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(with)Tj
1.9913 0 Td
(manufact)Tj
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(urers')Tj
2.7142 0 Td
(references)Tj
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(and)Tj
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(dilutions)Tj
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(used.)Tj
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-0.005 Tc
0 -2.1898 Td
(doi:)Tj
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(10.137)Tj
4.0323 0 Td
(1/journal.pone.)Tj
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(0170083.t)Tj
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(001)Tj
ET
Q
q
1 j
1 J
0 w
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