Green synthesis of silver nanoparticles by haloarchaeon Halococcus salifodinae BK6

Srivastava, Pallavee, Braganca, Judith, Ramanan, Sutapa Roy and Kowshik, Meenal 2014. Green synthesis of silver nanoparticles by haloarchaeon Halococcus salifodinae BK6. Presented at: International Conference on Nano Materials: Science, Technology and Applications 2013 (ICNM), Vandalur, India, 05-07 December 2013. Advanced Materials Research. , vol.938 Trans Tech Publications, pp. 236-241. 10.4028/www.scientific.net/AMR.938.236

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Abstract

Nanobiotechnology is a multidisciplinary branch of nanotechnology which includes fabrication of nanosized materials using biological approaches. Highly structured metallic and metal sulfide nanoparticles have been reported to be synthesized by numerous bacteria, fungi, yeasts and viruses. However, biosynthesis of nanoparticles by Haloarchaea (salt-loving archaea) of the third domain of life, Archaea, is in its nascent stages. In this study, we report the intracellular synthesis of stable, mostly spherical silver nanoparticles (SNPs) by the haloarchaeal isolate Halococcus salifodinae BK6. The isolate adapted to silver nitrate was found to exhibit growth kinetics similar to that of cells unexposed to silver nitrate. The nitrate reductase enzyme assay and the enzyme inhibitor studies showed the involvement of NADH dependent nitrate reductase in silver tolerance, reduction, and synthesis of SNPs. UV visible spectroscopy, XRD, TEM and EDAX were used for characterization of SNPs. The XRD exhibited characteristic Bragg peaks of face centered cubic silver with crystallite domain size of 26 nm and 12 nm for SNPs synthesized in NTYE and halophilic nitrate broth, respectively. TEM analysis exhibited an average particle size of 50.3 nm and 12 nm for SNPs synthesized in NTYE and halophilic nitrate broth (HNB), respectively. The as synthesized SNPs exhibited antimicrobial activity against both Gram positive and Gram negative organisms.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Published Online
Status:	Published
Schools:	Engineering
Publisher:	Trans Tech Publications
ISSN:	1662-8985
Funders:	Ministry of Earth Sciences
Last Modified:	27 Jul 2021 14:00
URI:	https://orca.cardiff.ac.uk/id/eprint/142745

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