Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Chronic hypoxia up-regulates expression of adenosine A1 receptors in DDT1-MF2 cells

Hammond, Lucy C., Bonnet, Claire, Kemp, Paul J. ORCID: https://orcid.org/0000-0003-2773-973X, Yates, Michael S. and Bowmer, Christopher J. 2004. Chronic hypoxia up-regulates expression of adenosine A1 receptors in DDT1-MF2 cells. Biochemical Pharmacology 67 (3) , pp. 421-426. 10.1016/j.bcp.2003.09.003

Full text not available from this repository.

Abstract

As the first step to understand how chronic hypoxia might regulate smooth muscle function in health and disease, we have employed an established immortalised cell model of smooth muscle, DDT1-MF2 cells, to address the hypothesis that adenosine A1 receptor density is modulated by O2 availability. Maximal specific binding (Bmax) of the selective adenosine A1 receptor antagonist, []-DPCPX, to cell membranes increased 3.5-fold from 0.48±0.02 pmol/mg to 1.7±0.5 pmol/mg protein after 16 hr of hypoxia and this effect was not accompanied by any statistically significant changes in either binding affinity (0.84±0.2 nM vs. 1.2±0.3 nM) or Hill coefficient (1.1±0.1 vs. 0.99±0.03). Hypoxia-evoked increases in membrane receptor density were paralleled in intact DDT1-MF2 cells. In addition, the increase in []-DPCPX binding to intact cells was inhibited by co-incubation during hypoxia with the translational inhibitor cycloheximide, the transcriptional blocker actinomycin D and the NFκB inhibitor sulphasalazine. Together, these data show that adenosine A1 receptor density is modulated, at least in part, by O2-dependent activation of the transcription factor NFκB and adds to the list of processes dynamically regulated by ambient oxygen availability. Since hypoxia is an initiating factor in acute renal failure, similar changes in transcription may account for up-regulation of adenosine A1 receptors noted previously in the renal vasculature of rats with acute renal failure.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0006-2952
Last Modified: 27 Oct 2022 08:47
URI: https://orca.cardiff.ac.uk/id/eprint/63414

Citation Data

Cited 14 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item