Normal epithelial cells trigger EphA2-dependent RasV12 cell repulsion at the single cell level

Hill, William and Hogan, Catherine ORCID: https://orcid.org/0000-0002-1012-0896 2019. Normal epithelial cells trigger EphA2-dependent RasV12 cell repulsion at the single cell level. Small GTPases 10 (4) , pp. 305-310. 10.1080/21541248.2017.1324940

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Abstract

Epithelial cells expressing oncogenic Ras (RasV12) are detected by normal neighbors and are often extruded from tissues. We recently demonstrated that differential EphA2 signaling drives the segregation of mutant cells from normal monolayers via cell repulsion and increased RasV12 cell contractility. EphA2 signaling on RasV12 cells is triggered by ephrin-A ligands presented by normal cells. Here, we show that normal epithelial cells trigger the repulsion and enhanced contractility of Ras-transformed epithelial cells at the single cell level. We also reveal that ephrin-A ligands expressed on RasV12 cells are not required to drive RasV12 cell segregation following interaction with normal cells. Thus, normal-RasV12 cell-cell interaction triggers EphA2 forward signaling in RasV12 cells to drive repulsion and segregation of the transformed cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Publisher:	Taylor and Francis
ISSN:	2154-1248
Date of First Compliant Deposit:	27 April 2017
Date of Acceptance:	26 April 2017
Last Modified:	30 Jun 2023 21:56
URI:	https://orca.cardiff.ac.uk/id/eprint/100164

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