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Using phage display technology to obtain Crybodies active against non-target insects

Dominguez-Flores, Tania, Romero-Bosquet, Maria Dolores, Gantiva-Diaz, Diana Marcela, Luque-Navas, Maria Jose, Berry, Colin, Osuna, Antonio and Vilchez, Susana 2017. Using phage display technology to obtain Crybodies active against non-target insects. Scientific Reports 7 , 14922. 10.1038/s41598-017-09384-x

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The insecticidal Cry toxins produced by Bacillus thuringiensis (Bt) are increasingly important in the biological control of insect pests and vectors of human disease. Markets for Bt products and transgenic plants expressing their toxins are driven by their specificity, safety and the move away from chemical control agents. However, the high specificity of Cry toxins can also prove to be a limitation when there is no known Cry toxin active against a particular target. Novel activities can be discovered by screening natural Bt isolates or through modifications of the Cry proteins. Here we demonstrate the use of λ-phage displaying Cry1Aa13 toxin variants modified in domain II loop 2 (Crybodies) to select retargeted toxins. Through biopanning using gut tissue from larvae of the non-target insect Aedes aegypti, we isolated a number of phage for further testing. Two of the overexpressed Cry toxin variants showed significant activity against A. aegypti larvae while another induced mortality at the pupal stage. We present the first report of the use of phage display to identify novel activities toward insects from distant taxonomic Orders and establish this technology based on the use of Crybodies as a powerful tool for developing tailor-made insecticides against new target insects.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.
Publisher: Nature Publishing Group
ISSN: 2045-2322
Date of First Compliant Deposit: 31 July 2017
Date of Acceptance: 26 July 2017
Last Modified: 09 Nov 2020 16:00

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