B cell activation and proliferation increase intracellular zinc levels

Ollig, Johanna, Kloubert, Veronika, Taylor, Kathryn ORCID: https://orcid.org/0000-0002-9576-9490 and Rink, Lothar 2019. B cell activation and proliferation increase intracellular zinc levels. Journal of Nutritional Biochemistry 64 , pp. 72-79. 10.1016/j.jnutbio.2018.10.008

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Abstract

Zinc ions serve as second messengers in major cellular pathways, including the regulation pathways of proliferation and their proper regulation is necessary for homeostasis and a healthy organism. Accordingly, expression of zinc transporters can be altered in various cancer cell lines and is often involved in producing elevated intracellular zinc levels. In this study, human B cells were infected with Epstein–Barr virus (EBV) to generate immortalized cells, which revealed traits of tumor cells, such as high proliferation rates and an extended lifespan. These cells showed differentially altered zinc transporter expression with ZIP7 RNA and protein expression being especially increased as well as a corresponding increased phosphorylation of ZIP7 in EBV-transformed B cells. Accordingly, free zinc levels were elevated within these cells. To prove whether the observed changes resulted from immortalization or rather high proliferation, free zinc levels in in vitro activated B cells and in freshly isolated B cells expressing the activation marker CD69 were determined. Here, comparatively increased zinc levels were found, suggesting that activation and proliferation, but not immortalization, act as crucial factors for the elevation of intracellular free zinc.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Publisher:	Elsevier
ISSN:	0955-2863
Funders:	Wellcome Trust
Date of First Compliant Deposit:	23 November 2018
Date of Acceptance:	17 October 2018
Last Modified:	05 May 2023 07:03
URI:	https://orca.cardiff.ac.uk/id/eprint/117065

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