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Rapid DNA analysis for automated processing and interpretation of low DNA content samples

Turingan, Rosemary S., Vasantgadkar, Sameer, Palombo, Luke, Hogan, Catherine, Jiang, Hua, Tan, Eugene and Selden, Richard F. 2016. Rapid DNA analysis for automated processing and interpretation of low DNA content samples. Investigative Genetics 7 (1) , 2. 10.1186/s13323-016-0033-7

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Short tandem repeat (STR) analysis of casework samples with low DNA content include those resulting from the transfer of epithelial cells from the skin to an object (e.g., cells on a water bottle, or brim of a cap), blood spatter stains, and small bone and tissue fragments. Low DNA content (LDC) samples are important in a wide range of settings, including disaster response teams to assist in victim identification and family reunification, military operations to identify friend or foe, criminal forensics to identify suspects and exonerate the innocent, and medical examiner and coroner offices to identify missing persons. Processing LDC samples requires experienced laboratory personnel, isolated workstations, and sophisticated equipment, requires transport time, and involves complex procedures. We present a rapid DNA analysis system designed specifically to generate STR profiles from LDC samples in field-forward settings by non-technical operators. By performing STR in the field, close to the site of collection, rapid DNA analysis has the potential to increase throughput and to provide actionable information in real time.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: BioMed Central
ISSN: 2041-2223
Date of First Compliant Deposit: 7 January 2019
Date of Acceptance: 26 February 2016
Last Modified: 10 Dec 2019 15:41

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