Stuerzenbaum, Stephen R., Georgiev, Oleg, Morgan, Andrew John and Kille, Peter ORCID: https://orcid.org/0000-0001-6023-5221 2004. Cadmium detoxification in earthworms: from genes to cells. Environmental Science and Technology 38 (23) , pp. 6283-6289. 10.1021/es049822c |
Abstract
Cadmium pollution has clear ecotoxicological consequences as it is readily bio-available and has a reported tendency to bio-accumulate in soil biota. Understanding the mechanisms of cadmium trafficking pathways within sentinel terrestrial invertebrates, such as the earthworm, is therefore considered to be of importance. Using X-ray microanalysis, quantitative polymerase chain reaction, and immunohistochemical techniques, we were able to demonstrate that the earthworm has the intrinsic capacity to efficiently sequester and compartmentalize cadmium via a metallothionein-mediated trafficking pathway. There is evidence that wMT-2, rather than wMT-1, is the major isoform implicated in the detoxification of cadmium and the identification of three independent wMT-2 loci (totalling over 25 kb of genomic sequence) has revealed a complex genomic organization. Complementary in silico analysis of over 6500 expressed sequence tags has identified a third metallothionein isoform, wMT-3, found to be highly enriched in embryonic tissue. In summary, this paper provides a detailed dissection of the genetic, molecular, and cellular basis of a sophisticated pathway that facilitates the uptake, accumulation, transport, and excretion of cadmium.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Biosciences |
ISSN: | 0013-936X |
Last Modified: | 17 Oct 2022 08:50 |
URI: | https://orca.cardiff.ac.uk/id/eprint/1200 |
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