Gompertzian growth pattern correlated with phenotypic organization of colon carcinoma, malignant glioma and non-small cell lung carcinoma cell lines

Castro, M. A. A., Klamt, F., Grieneisen, V. A. ORCID: https://orcid.org/0000-0001-6780-8301, Grivicich, I. and Moreira, J. C. F. 2003. Gompertzian growth pattern correlated with phenotypic organization of colon carcinoma, malignant glioma and non-small cell lung carcinoma cell lines. Cell Proliferation 36 (2) , pp. 65-73. 10.1046/j.1365-2184.2003.00259.x

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Abstract

In the current study we present a Gompertzian model for cell growth as a function of cell phenotype using six human tumour cell lines (A‐549, NCI‐H596, NCI‐H520, HT‐29, SW‐620 and U‐251). Monolayer cells in exponential growth at various densities were quantified over a week by sulforhodamine B staining assay to produce cell‐growth curves. A Gompertz equation was fitted to experimental data to obtain, for each cell line, three empirical growth parameters (initial cell density, cell‐growth rate and carrying capacity – the maximal cell density). A cell‐shape parameter named deformation coefficient D (a morphological relationship among spreading and confluent cells) was established and compared by regression analysis with the relative growth rate parameter K described by the Gompertz equation. We have found that coefficient D is directly proportional to the growth parameter K. The fit curve significantly matches the empirical data (P < 0.05), with a correlation coefficient of 0.9152. Therefore, a transformed Gompertzian growth function was obtained accordingly to D. The degree of correlation between the Gompertzian growth parameter and the coefficient D allows a new interpretation of the growth parameter K on the basis of morphological measurements of a set of tumour cell types, supporting the idea that cell‐growth kinetics can be modulated by phenotypic organization of attached cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Wiley
ISSN:	0960-7722
Date of Acceptance:	10 January 2003
Last Modified:	25 Oct 2022 13:41
URI:	https://orca.cardiff.ac.uk/id/eprint/120505

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