Nuclear migration: an indicator of plant salinity tolerance in vitro

Elmaghrabi, Adel M., Francis, Dennis, Rogers, Hilary J. ORCID: https://orcid.org/0000-0003-3830-5857 and Ochatt, Sergio J. 2019. Nuclear migration: an indicator of plant salinity tolerance in vitro. Frontiers in Plant Science 10 , 783. 10.3389/fpls.2019.00783

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Abstract

In order to understand the mechanisms underlying acquisition of tolerance to salinity, we recently produced callus tissues of tobacco and Medicago truncatula resistant to NaClinduced salt stress following application of a step-up recurrent selection method. The effects of salinity on cell size are known, but those on cell morphometry including cell and nuclear surface area and position of nuclei within salt stress resistant cells were never studied before. This work fills that gap, using suspension cultured cells of M. truncatula A17 initiated from callus, and Nicotiana tabacum BY-2 cell line resistant to increasing NaCl concentrations up to 150 mM NaCl. The surface area of salinity resistant cells of M. truncatula A17 and N. tabacum BY2 and their nuclei, produced by step-up recurrent selection, were reduced, and cells elongated as NaCl increased, but these parameters proved to be unreliable in explaining cell survival and growth at high NaCl. Conversely, nuclei of resistant cells migrated from the center to the periphery of the cytoplasm close to the walls. Nuclear marginalization was for the first time observed as a result of salt stress in plant cells, and could be a novel helpful morphological marker of acquisition of salinity tolerance.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Frontiers Media
ISSN:	1664-462X
Date of First Compliant Deposit:	29 May 2019
Date of Acceptance:	29 May 2019
Last Modified:	03 May 2023 15:13
URI:	https://orca.cardiff.ac.uk/id/eprint/122946

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