Disruption of Micro-organisms

Coakley, W.T., Bater, A.J. and Lloyd, D. ORCID: https://orcid.org/0000-0002-5656-0571 1977. Disruption of Micro-organisms. Advances in Microbial Physiology 16 (C) , pp. 279-341. 10.1016/S0065-2911(08)60050-8

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Abstract

Techniques for the disruption of cells have evolved and for the most part suitable methods for the solution of individual needs have been arrived at by a pragmatic approach. It is clear, however, that from the viewpoint of future developments much is to be gained from a rigorous physical and biochemical understanding of the processes involved in cell breakage. In recent years, a requirement for a greater degree of sophistication has become evident as the emphasis has shifted from the aim of quantitative enzyme release to that of the preservation of information content within the disrupted system. Many of the problems of the efficient extraction of intracellular components have been overcome via a diversity of methodological developments, it is evident that the current demands made on disruption procedures on the part of microbial physiologists engaged in fundamental studies of sub-cellular organization can only be partially satisfied. Extrapolation of data obtained with cell-free extracts to the in vivo situation requires independent confirmation. . Many of the methods used for quantitative release of enzymes from micro-organisms, especially those relying on the development of high liquid or solid shearing forces are not suitable for the release of “intact” membranes or organelles. As further information on the complexity of intracellular organization becomes available, it becomes increasingly evident that the extraction of “intact” organelles may often be an unattainable goal.

Item Type:	Article
Status:	Published
Schools:	Biosciences
Last Modified:	26 Oct 2022 08:32
URI:	https://orca.cardiff.ac.uk/id/eprint/127720

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