Simultaneous dissolved oxygen and redox measurements: Use of polarographic, bioluminescence and mass spectrometric monitoring combined with dual-wavelength spectrophotometry

Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571 1985. Simultaneous dissolved oxygen and redox measurements: Use of polarographic, bioluminescence and mass spectrometric monitoring combined with dual-wavelength spectrophotometry. Presented at: Symposium on Gas Enzymology, Odense, Denmark, 28–29 May 1984. Published in: Degn, H., Cox, R. P. and Toftlund, H. eds. Gas Enzymology. Dordrecht: D. Reidel Publishing Company, pp. 37-53. 10.1007/978-94-009-5279-9_3

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Abstract

Comparison of available methods for O2 determinations reveals that of the few ways of measuring dissolved O2, the polarographic approach as employed in a continuously-stirred reactor open to gases is extremely useful. However it is complemented and extended in terms of versatility and stability by membrane inlet mass spectrometry, and for sensitivity by the bioluminescence method. Combined with dual wavelength spectrophotometry, fluorimetry or chemiluminescence, these experimental systems provide a powerful approach to problems of microbial and cell biochemistry, as they provide continuous readout of direct non-invasive measurements of functions in intact organisms. Applications are presented: studies of O2 effects on microorganisms with an obligate requirement for oxygen (amoeba), a facultative anaerobe (yeast) and aerotolerant and strict anaerobes (rumen and anaerobic digester populations respectively).

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	D. Reidel Publishing Company
ISBN:	978-94-010-8831-2
Last Modified:	26 Oct 2022 08:32
URI:	https://orca.cardiff.ac.uk/id/eprint/127735

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