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Respiration of Trichomonas vaginalis Components detected by electron paramagnetic resonance spectroscopy

Chapman, A., Cammack, R., Linstead, D.J. and Lloyd, D. ORCID: https://orcid.org/0000-0002-5656-0571 1986. Respiration of Trichomonas vaginalis Components detected by electron paramagnetic resonance spectroscopy. European Journal of Biochemistry 156 (1) , pp. 193-198. 10.1111/j.1432-1033.1986.tb09567.x

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Abstract

1 Electron paramagnetic resonance (EPR) spectrometry was used to characterise the iron‐sulphur clusters present in the human parasite, Trichomonas vaginalis, an aerotolerant anaerobic protozoan which lacks mitochondria. 2 From observations of whole cells and subcellular fractions, the majority of the EPR signals are derived from one of the two main terminal respiration sites, the hydrogenosome; fractionation of hydrogenosomes revealed that a large proportion of these signals were associated with the membrane of these organelles. 3 Resolution into eight species was achieved by varying the degree of reduction and the temperature of the samples. 4 One component, a [2Fe–2S] ferredoxin reducible by pyruvate: ferredoxin oxidoreductase, corresponds to that previously purified, and predominates in spectra scanned at temperatures higher than 45 K. This species (mid‐point redox potential – 300 ± 20 mV) was detected both in a hydrogenosomal membrane fraction and in the matrix fraction derived from broken organelles. 5 Another iron‐sulphur species (mid‐point potential – 270 mV) was detectable in both membrane and matrix at temperatures lower than 30 K. 6 Four other reduced species were confined to the hydrogenosomal membrane and a fifth was not detected after subfractionation, although it appeared in intact organelles. This species is assumed to be highly unstable. 7 Free‐radical signals in hydrogenosome‐enriched fractions probably arise from flavoprotein semiquinones; no other signals were obtained from the cytosolic fractions incubated with NADH. 8 A signal in the oxidised state was observed in the hydrogenosomal membrane. 9 Possible identities of hydrogenosomal iron‐sulphur clusters are discussed in relation to previously established enzyme activities.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Wiley: No OnlineOpen
ISSN: 0014-2956
Last Modified: 26 Oct 2022 08:33
URI: https://orca.cardiff.ac.uk/id/eprint/127765

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