Rapid estimation of bacterial antibiotic susceptibility with flow cytometry

MASON, D.J., ALLMAN, R., STARK, J.M. and LLOYD, D. ORCID: https://orcid.org/0000-0002-5656-0571 1994. Rapid estimation of bacterial antibiotic susceptibility with flow cytometry. Journal of Microscopy 176 (1) , pp. 8-16. 10.1111/j.1365-2818.1994.tb03494.x

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Abstract

Bacterial antibiotic susceptibility was rapidly estimated for Escherichia coli and Staphylococcus spp. by flow cytometry. This was achieved by measuring the uptake of a negatively charged membrane potential sensitive dye bis‐(1,3‐dibutylbarbituric acid) trimethine oxonol and observing changes in low‐angle light scatter (excitation light scattered by up to 15°). Estimations of ampicillin, gentamicin and ciprofloxacin susceptibilities were possible within 2–5 h from a plate culture, depending on the species and antibiotic used. This includes the time necessary to establish steady‐state growth in liquid culture.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Wiley
ISSN:	0022-2720
Date of Acceptance:	9 August 1994
Last Modified:	26 Oct 2022 08:35
URI:	https://orca.cardiff.ac.uk/id/eprint/127846

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