The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria.

Mason, D J, Lopez-Amoros, R, Allman, R, Stark, J M and Lloyd, D ORCID: https://orcid.org/0000-0002-5656-0571 1995. The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria. JOURNAL OF APPLIED BACTERIOLOGY 78 (3) , 309--315. 10.1111/j.1365-2672.1995.tb05031.x

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Abstract

Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
ISSN:	0021-8847
Date of Acceptance:	4 November 1994
Last Modified:	26 Oct 2022 08:35
URI:	https://orca.cardiff.ac.uk/id/eprint/127853

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