Carbon monoxide- and oxygen-reacting haemoproteins in the mitochondrial fraction from the soil amoeba Acanthamoeba castellanii: Studies at subzero temperatures.

Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571, Edwards, Steven W. and Chance, Britton 1981. Carbon monoxide- and oxygen-reacting haemoproteins in the mitochondrial fraction from the soil amoeba Acanthamoeba castellanii: Studies at subzero temperatures. The Biochemical Journal 200 (2) , pp. 337-342. 10.1042/bj2000337

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Abstract

1. Mitochondria-enriched fractions of the soil amoeba Acanthamoeba castellanii contained four haemoproteins that in their reduced forms reacted with CO to give photodissociable CO complexes; these were cytochromes a 3, a 614, b- and c-type cytochromes. 2. Non-photodissociable oxygen-containing compounds were formed at temperatures between -130 and -150 degrees C after photodissociation of CO in the presence of 200 microM-O2, 3. Electron transport, indicated by the oxidation of cytochromes a + a3 and cytochrome c, did not occur until the temperature was raised to -80 degrees C.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
ISSN:	0264-6021
Date of Acceptance:	4 August 1981
Last Modified:	26 Oct 2022 08:37
URI:	https://orca.cardiff.ac.uk/id/eprint/127934

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