Oxygen affinity of the respiratory chain of Acanthamoeba castellanii

Lloyd, D. ORCID: https://orcid.org/0000-0002-5656-0571, Mellor, H. and Williams, J. L. 1982. Oxygen affinity of the respiratory chain of Acanthamoeba castellanii. Biochemical Journal 214 , pp. 47-51. 10.1042/bj2140047

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Abstract

Apparent Km values for O2 for the soil amoeba Acanthamoeba castellanii determined polarographically and by bioluminescence gave similar values (0.37 and 0.41 microM respectively). Mitochondria oxidizing succinate or NADH in the presence or absence of ADP gave values in the range 0.21-0.36 microM-O2. Oxidation of respiratory-chain components to 50% of the aerobic steady states in intact cells was observed at the following O2 concentrations: cytochrome aa3, 0.1-0.25 microM; cytochrome c, 0.3-0.6 microM; cytochrome b, 0.35-0.45 microM; flavoprotein, 2 microM. In isolated mitochondria corresponding values for a-, c- and b-type cytochromes were 0.007, 0.035-0.05 and 0.06-0.09 microM-O2. It is concluded that an O2 gradient exists between plasma membrane and mitochondria in A. castellanii.

Item Type:	Article
Date Type:	Submission
Status:	Published
Schools:	Biosciences
Publisher:	Portland Press
ISSN:	0264-6021
Date of Acceptance:	29 March 1983
Last Modified:	26 Oct 2022 08:38
URI:	https://orca.cardiff.ac.uk/id/eprint/127946

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