The Pasteur effect in yeasts: mass spectrometric monitoring of oxygen uptake, and carbon dioxide and ethanol production

Lloyd, D ORCID: https://orcid.org/0000-0002-5656-0571 and James, CJ 1987. The Pasteur effect in yeasts: mass spectrometric monitoring of oxygen uptake, and carbon dioxide and ethanol production. FEMS Microbiology Letters 42 (1) , 27--31. 10.1111/j.1574-6968.1987.tb02294.x

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Abstract

Simultaneous and continuous monitoring of dissolved O2, CO2 and ethanol in suspensions of Saccharomyces uvarum and Saccharomyces cerevisiae, by means of a quadrupole mass spectrometer fitted with a silicone rubber membrane inlet, enabled a study of O2 inhibition of glycolysis (Pasteur effect). Calculation of Pasteur quotients (ratios of anaerobic to aerobic glycolytic rates) give similar values when corrected both for respiratory CO2 production and ethanol oxidation under aerobic conditions. For S. cerevisiae the apparent Km for O2 of the respiration of catabolite derepressed cells was 1 μM and the apparent Ki values for O2 as an inhibitor of glycolysis were 12.7 and 7 μM, respectively, when measurements were made on CO2 evolution and ethanol production.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Oxford University Press (OUP): Policy B - Oxford Open Option D
ISSN:	0378-1097
Date of Acceptance:	9 January 1987
Last Modified:	26 Oct 2022 08:39
URI:	https://orca.cardiff.ac.uk/id/eprint/127987

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