Effect of 2-deoxy-d-glucose on growth and cell walls of Schizosaccharomyces pombe 972h-

Poole, R K and Lloyd, D ORCID: https://orcid.org/0000-0002-5656-0571 1973. Effect of 2-deoxy-d-glucose on growth and cell walls of Schizosaccharomyces pombe 972h-. Archiv für Mikrobiologie 88 (4) , 257--272. 10.1007/BF00409939

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Abstract

The effect of 2-deoxy-D-glucose on growth of Schizosaccharomyces pombe 972h−, and on its cell wall composition, structure and susceptibility to enzymatic degradation has been investigated. The growth rate was not markedly affected at below 80 μg/ml of the inhibitor, but the increased frequency of appearance of aberrant forms and the reduction in the final population attained varied directly with increased inhibitor concentration. These abnormal cells show localized lesions and, at these points, extrusion of cell contents still enclosed by an inner cell wall layer which is not sensitive to osmotic shock. Cells grown normally yield “prosphaeroplasts” on treatment with snail digestive enzymes; thiol pretreatment was not necessary but degradation proceeded more rapidly when MgSO4 was used in place of sorbitol as osmotic stabilizer. Deoxyglucose-grown organisms rapidly yield true osmotically-sensitive sphaeroplasts on similar treatment.Analysis of isolated walls from mechanically disrupted cells showed marked changes in composition; an increase in the total lipid was accompanied by decreased content of both glucose and glucosamine.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Last Modified:	26 Oct 2022 08:40
URI:	https://orca.cardiff.ac.uk/id/eprint/128006

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