Methanogenesis from methanol in Methanosarcina barkeri studied using membrane inlet mass spectrometry

Ranalli, G., Whitmore, T. N. and Lloyd, D. ORCID: https://orcid.org/0000-0002-5656-0571 1986. Methanogenesis from methanol in Methanosarcina barkeri studied using membrane inlet mass spectrometry. FEMS Microbiology Letters 35 (2-3) , p. 119. 10.1111/j.1574-6968.1986.tb01512.x

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Abstract

A mass spectrometer with membrane inlet was used to study methanol metabolism by Methanosarcina barkeri strain MS. The addition of methanol to methanol grown culture samples in the mass spectrometer vessel stimulated methanogenesis and hydrogen production. The apparent Ks for methanol was determined as 0.5 mM and the Vmax as 8.14 mmol g (dry weight) h−1. The Vmax for methane production was fairly constant during growth of the culture on methanol implying that growth is tightly coupled to methanogenesis. The addition of methanol to culture samples in the mass spectrometer vessel stimulated methanogenesis with no lag which indicated that methanogenesis can be uncoupled from growth. Exposure of the culture sample in the mass spectrometer vessel to an atmosphere of 2 kPa oxygen for 80 min resulted in a decrease in the rate of methanogenesis from methanol but on returning the atmosphere to nitrogen the addition of further methanol stimulated methanogenesis. The effect of other inhibitors of methanogenesis (2-bromoethane sulphonate and monensin); Kj values 21.5 μM and 0.3 mM, respectively) were also studied.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Oxford University Press (OUP): Policy B - Oxford Open Option D
ISSN:	0378-1097
Date of Acceptance:	25 January 1986
Last Modified:	26 Oct 2022 08:40
URI:	https://orca.cardiff.ac.uk/id/eprint/128011

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