Thomas, K, Rutter, A, Suller, M, Harwood, J and Lloyd, D ORCID: https://orcid.org/0000-0002-5656-0571 1998. Oxygen induces fatty acid (n-6)-desaturation independently of temperature in Acanthamoeba castellanii. FEBS Letters 425 (1) , 171--174. 10.1016/s0014-5793(98)00218-x |
Abstract
Induction of a microsomal oleate delta12 (n-6) desaturase which is mainly responsible for an increase in membrane lipid unsaturation at low temperature has been observed in the free-living amoeba Acanthamoeba castellanii. In this study we show that the enzyme can also be regulated by oxygen independently of temperature in batch cultures grown to O2-limitation. Raising the oxygen concentration from below the lower limit of detection (< 0.1 microM) to approximately air-saturation (230 microM), whilst maintaining the growth temperature constant (30 degrees C), increased lipid unsaturation and elevated n-6-desaturase activity 2.3-fold. Addition of the protein synthesis inhibitor, anisomycin, showed that increased desaturase activity was due to new protein synthesis rather than activation of pre-existing enzyme. These observations are important for future studies of the mechanism of temperature adaptation in poikilotherms.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Biosciences |
Publisher: | Elsevier |
ISSN: | 0014-5793 |
Last Modified: | 26 Oct 2022 08:40 |
URI: | https://orca.cardiff.ac.uk/id/eprint/128037 |
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