Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils

Hallett, Maurice B. ORCID: https://orcid.org/0000-0001-8197-834X, Roberts, Rhiannon E. and Dewitt, Sharon ORCID: https://orcid.org/0000-0001-8169-8241 2019. Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils. Neutrophil, Vol. 2087. Methods in Molecular Biology, New York: Humana Press, pp. 191-205. (10.1007/978-1-0716-0154-9_14)

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Abstract

The measurement and manipulation of cytosolic free Ca2+ of neutrophils is crucial for investigating the mechanisms within living neutrophils which generate Ca2+ signals and the cellular responses triggered by them. Optical methods for this are the most applicable for neutrophils, and are discussed here, especially the use of fluorescent indicators of Ca2+ and photoactivation of reagents involved in Ca2+ signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we outline some of the techniques that have been used to monitor, visualize, and manipulate Ca2+ in neutrophils.

Item Type:	Book Section
Date Type:	Published Online
Status:	Published
Schools:	Dentistry Medicine
Publisher:	Humana Press
ISBN:	9781071601532
ISSN:	1064-3745
Last Modified:	25 Nov 2022 12:14
URI:	https://orca.cardiff.ac.uk/id/eprint/128192

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