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New methods for in-situ quantitative measurement of dentine tubule occlusion

Olley, Ryan, Wilson, Ron, Parkinson, Charles, Moazzez, Rebecca and Bartlett, David 2012. New methods for in-situ quantitative measurement of dentine tubule occlusion. Presented at: 2012 IADR Pan European Region Meeting, Helisinki, Finland, 12-15 September 2012. SAGE Publications,

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Abstract

Objectives: Measurement of patent or un-occluded dentine tubules is often employed to evaluate the potential e!cacy of dentifrices designed to provide relief from dentine hypersensitivity via an occluding mode of action. This study aimed to create an innovative, automated computerised technique, to accurately and objectively quantify dentine tubule occlusion using novel methodological and computational procedures. Methods: Methods: Dentine samples (n=469), employed in an ethically approved in-situ clinical study involving 30 subjects (mean age 34.7 years) over four days, were used to investigate the level of tubule occlusion produced by two occlusion-based dentifrices containing 5% Novamin versus a control dentifrice containing NaF (1450ppm) or water. The samples were imaged pre and post treatment by Tandem Scanning Microscopy (TSM) and post treatment using Scanning Electron Microscopy (SEM). Tubule occlusion was determined using (i) a conventional approach of visually grading the amount of un-occluded dentine tubules on SEM images by three examiners using a visual ordinal scale (‘standard’) or (ii) computational software assessment of tubule patency with computer software speci"cally written using Image J (1.45s) to count the number of un-occluded dentine tubules. Results: Results: Statistically signi"cant positive Spearman correlations existed between the ‘standard’ and the software designed for SEM (0.58) and TSM (0.42) (p<0.001) (n=469). SEM visual grading and TSM computational analysis were both able to di#erentiate between the dentifrice’s formulated to produce occlusion of the dentine tubules and the control dentifrice or water (p<0.001). Intra-class correlation of the number of patent and un-occluded tubules counted visually and then by the software on thirty randomly chosen SEM or TSM images was 0.77 and 0.90 respectively. Conclusions: Conclusions: This study has identi"ed a novel computational-based image analysis routine capable of analysing dentine tubule occlusion quantitatively that may be used pre and post sample treatment. This routine allows for reproducible and objective assessment of dentine specimens.

Item Type: Conference or Workshop Item (Poster)
Date Type: Publication
Status: Published
Schools: Dentistry
Publisher: SAGE Publications
ISSN: 0022-0345
Last Modified: 17 Jun 2020 12:45
URI: http://orca.cardiff.ac.uk/id/eprint/132513

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