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Antibacterial and anti-virulence activity of manuka honey against genetically diverse Staphylococcus pseudintermedius

Brown, Helen L, Metters, Georgie, Hitchings, Matthew D, Wilkinson, Thomas S., Sousa, Luis, Cooper, Jenna, Dance, Harry, Atterbury, Robert J. and Jenkins, Rowena 2020. Antibacterial and anti-virulence activity of manuka honey against genetically diverse Staphylococcus pseudintermedius. Applied and Environmental Microbiology 86 , e01768-20. 10.1128/AEM.01768-20

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Abstract

Staphylococcus pseudintermedius causes opportunistic infections in dogs. It also has significant zoonotic potential, with the emergence of multidrug resistance leading to difficulty treating both animal and human infections. Manuka honey has previously been reported to inhibit many bacterial pathogens, including methicillin-resistant Staphylococcus aureus, and is successfully utilized in both clinical and veterinary practice. Here, we evaluated the ability of manuka honey to inhibit strains of S. pseudintermedius grown alone and in combination with antibiotics, as well as its capacity to modulate virulence within multiple S. pseudintermedius isolates. All 18 of the genetically diverse S. pseudintermedius strains sequenced and tested were inhibited by ≤12% (wt/vol) medical-grade manuka honey, although tolerance to five clinically relevant antibiotics was observed. The susceptibility of the isolates to four of these antibiotics was significantly increased (P ≤ 0.05) when combined with sublethal concentrations of honey, although sensitivity to oxacillin was decreased. Virulence factor (DNase, protease, and hemolysin) activity was also significantly reduced (P ≤ 0.05) in over half of isolates when cultured with sublethal concentrations of honey (13, 9, and 10 isolates, respectively). These findings highlight the potential for manuka honey to be utilized against S. pseudintermedius infections.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Dentistry
Publisher: American Society for Microbiology
ISSN: 0099-2240
Funders: Waterloo Foundation
Date of First Compliant Deposit: 7 September 2020
Date of Acceptance: 14 August 2020
Last Modified: 05 Nov 2020 14:01
URI: http://orca.cardiff.ac.uk/id/eprint/134663

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