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Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean

Parmezan, Talitta Regina, Brito Júnior, Salvador Lima, Carvalho, Kenia de, Aquino, Moisés de, Birkett, Michael, Pickett, John, Nunes, Estela de Oliveira, Abdelnor, Ricardo Vilela, Campo, Clara Beatriz Hoffmann and Marcelino-Guimarães, Francismar Corrêa 2020. Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean. Genetics and Molecular Biology 43 (4) , e20190388. 10.1590/1678-4685-gmb-2019-0388

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Abstract

Terpenes produced by plants comprise a diverse range of secondary metabolites, including volatile organic compounds (VOCs). Terpene VOC production may be altered after damage or by biological stimuli such as bacterial, fungal and insects, and subsequent triggering of plant defense responses. These VOCs originate in plants from two independent pathways: the mevalonate and the methylerythritol phosphate pathways, which utilize dimethylallyl and isopentenyl diphosphates to form the terpenoidal precursors. Phakopsora pachyrhizi fungi causes Asian soybean rust, limiting soybean production and resulting in losses of up to 80% if no control strategies are applied. By using a transcriptome datasets, we investigated the regulation of genes of the mevalonate pathway under different biotic stresses. We studied the impact of P. pachyrhizi infection in vivo expression profile of genes involved in terpenoid and glyceollin biosynthesis in genotypes harboring different resistance genes (Rpp), and across the infection cycle. In addition, we used UPLC and UPGC analysis to evaluate glyceollin and VOC production, respectively, to identify metabolites associated with soybean responses to pathogen infection. The regulation of soybean genes involved in terpene production was influenced by genotypes, depending on the Rpp gene, while glyceollin was induced in all genotypes. Furthermore, a sesquiterpene was identified as a potential marker associated with rust symptoms on soybean.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
ISSN: 1678-4685
Funders: BBSRC
Date of First Compliant Deposit: 11 November 2020
Date of Acceptance: 8 July 2020
Last Modified: 12 Nov 2020 10:00
URI: http://orca.cardiff.ac.uk/id/eprint/136256

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