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Development of nanosponges from erythrocyte ghosts for removal of streptolysin-O from mammalian blood

Chhabria, Vikesh and Beeton, Steve 2016. Development of nanosponges from erythrocyte ghosts for removal of streptolysin-O from mammalian blood. Nanomedicine 11 (21) , 2797–2807. 10.2217/nnm-2016-0180

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Abstract

Aim: To produce mammalian biomimetic nanosponges from mammalian erythrocyte ghosts. Biomimetic nanosponges were studied in vitro as treatment platforms against exotoxin-related sepsis. Methods: Ovine blood was treated with hypotonic buffer to create erythrocyte ghosts and then subjected to sonication to produce erythrocyte vesicles of nonuniform size. Vesicles were then serially extruded through 400-nm and 100-nm polycarbonate membranes. Nanosponges were prepared by fusing poly(d,l-lactic-co-glycolic acid) cores with ovine erythrocyte vesicles. Results: Ovine erythrocytes were the most susceptible to streptolysin-O lysis, making it a model to study sepsis treatment. Ovine nanosponges adsorbed streptolysin-O at 37 and 40°C. Conclusion: These results identify ovine nanosponges as novel therapeutic model to test adsorption of cholesterol binding toxins such as streptolysin-O.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Future Medicine
ISSN: 1743-5889
Date of First Compliant Deposit: 26 January 2021
Date of Acceptance: 27 June 2016
Last Modified: 27 Jan 2021 10:00
URI: http://orca.cardiff.ac.uk/id/eprint/137957

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