Maturation of stretch-induced contractile activity and its muscarinic regulation in isolated whole bladder strips from rat

Gevaert, Thomas, Owsianik, Grzegorz, Hutchings, Graham John ORCID: https://orcid.org/0000-0001-8885-1560, Everaerts, Wouter, Nilius, Bernd and De Ridder, Dirk 2010. Maturation of stretch-induced contractile activity and its muscarinic regulation in isolated whole bladder strips from rat. Neurourology and Urodynamics 29 (5) , pp. 789-796. 10.1002/nau.20553

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Abstract

Aim Besides the establishment of neural reflex pathways, developmental changes in local bladder properties probably also contribute to the onset of mature voiding reflexes. Here we explored the behavior of stretch-induced contractile activity (SIC) and its muscarinic regulation in neonatal and adult rat bladders. Methods SIC was studied in bladder strips from D0, D7, D28 rat pups and adult rats (15 weeks). The responses to a non-selective [Carbachol (CA), 10−8.5–10−6 M] and an M2-selective muscarinic agonist [arecaidine but-2-ynyl ester tosylate (ABET), 10−9.5–10−7 M] were studied. The expression of M2 and M3 mRNA was investigated using quantitative PCR in whole bladders. The response of SIC to KCl (50 mM) and to the non-adrenergic non-cholinergic (NANC) drugs Substance P (1 µM) and ,β-Methyleneadenosine 5′-triphosphate lithium salt (MATP) (1 µM) were also studied. Results We found evidence for an enhanced response to the muscarinic agonists CA and ABET in neonatal bladders. This might be due to the onset of a direct contractile role for M2, given the moderate M2-properties of ABET and the absence of ABET-effects on adult bladder strips. Further data showed an increased expression of both M2 and M3 at the mRNA level and a changed response of SIC to NANC drugs in neonatal bladder. Conclusions This study reveals a changed response of SIC to muscarinic and NANC drugs in neonatal rat bladder together with changes at the muscarinic mRNA level, which might all contribute to a better insight in the role of SIC in the onset of mature voiding. Neurourol. Urodynam. 29:789–796, 2010.

Item Type:	Article
Status:	Published
Schools:	Chemistry Cardiff Catalysis Institute (CCI)
Subjects:	Q Science > QD Chemistry
Uncontrolled Keywords:	bladder strips; muscarinic; M2; M3; neonatal bladder
Publisher:	Wiley-Liss
ISSN:	0733-2467
Last Modified:	18 Oct 2022 13:34
URI:	https://orca.cardiff.ac.uk/id/eprint/14379

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