The lung microbiota in children with cystic fibrosis captured by induced sputum sampling

Weiser, Rebecca ORCID: https://orcid.org/0000-0003-3983-3272, Oakley, Juliette, Ronchetti, Katherine, Tame, Jodee, Hoehn, Sven, Jurkowski, Tomasz P. ORCID: https://orcid.org/0000-0002-2012-0240, Mahenthiralingam, Eshwar ORCID: https://orcid.org/0000-0001-9014-3790 and Forton, Julian T. ORCID: https://orcid.org/0000-0002-0580-0432 2022. The lung microbiota in children with cystic fibrosis captured by induced sputum sampling. Journal of Cystic Fibrosis 21 (6) , pp. 1006-1012. 10.1016/j.jcf.2022.01.006

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Abstract

Background Spatial topography of the cystic fibrosis (CF) lung microbiota is poorly understood in childhood. How best to sample the respiratory tract in children for microbiota analysis, and the utility of microbiota profiling in clinical management of early infection remains unclear. By comparison with bronchoalveolar lavage (BAL), we assessed the ability of induced sputum (IS) sampling to characterise the lower airway microbiota. Methods Sample sets from IS and two or three matched BAL compartments were obtained for microbiota analysis as part of the CF-Sputum Induction Trial (UKCRN_14615, ISRCTNR_12473810). Microbiota profiles and pathogen detection were compared between matched samples. Results Twenty-eight patients, aged 1.1–17.7 years, provided 30 sample sets. Within-patient BAL comparisons revealed spatial heterogeneity in 8/30 (27%) sample sets indicating that the lower airway microbiota from BAL is frequently compartmentalised in children with CF. IS samples closely resembled one or more matched BAL compartments in 15/30 (50%) sets, and were related in composition in a further 9/30 (30%). IS detected 86.2% of the Top 5 genera found across matched BAL samples. The sensitivity of IS to detect specific CF-pathogens identified in matched BAL samples at relative abundance ≥5% varied between 43 and 100%, with negative predictive values between 73 and 100%. Conclusions Spatial heterogeneity of the lower airway microbiota was observed in BAL samples and presents difficulties for consistent lung sampling. IS captured a microbiota signature representative of the lower airway in 80% of cases, and is a straightforward, non-invasive intervention that can be performed frequently to aid pathogen diagnosis and understand microbiota evolution in children with CF.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Biosciences
Publisher:	Elsevier
ISSN:	1569-1993
Date of First Compliant Deposit:	11 January 2022
Date of Acceptance:	9 January 2022
Last Modified:	18 Aug 2023 08:13
URI:	https://orca.cardiff.ac.uk/id/eprint/146540

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