Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media

Tizaoui, Chedly, Stanton, Richard ORCID: https://orcid.org/0000-0002-6799-1182, Statkute, Evelina, Rubina, Anzelika, Lester-Card, Edward, Lewis, Anthony, Holliman, Peter and Worsley, Dave 2022. Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media. Journal of Hazardous Materials 428 , 128251. 10.1016/j.jhazmat.2022.128251

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Abstract

This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log10-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log10-reduction per ozone CT value(g min/m3) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Publisher:	Elsevier
ISSN:	0304-3894
Date of First Compliant Deposit:	17 January 2022
Date of Acceptance:	7 January 2022
Last Modified:	05 May 2023 15:08
URI:	https://orca.cardiff.ac.uk/id/eprint/146567

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