Growth Factor release and dental pulp stem cell attachment following dentine conditioning- an in vitro study

Sadaghiani, Leili, Alshumrani, Abdullah Mohammad, Gleeson, Hannah, Nishio Ayre, Wayne ORCID: https://orcid.org/0000-0003-2405-1876 and Sloan, Alastair ORCID: https://orcid.org/0000-0002-1791-0903 2022. Growth Factor release and dental pulp stem cell attachment following dentine conditioning- an in vitro study. International Endodontic Journal 10.1111/iej.13781

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Abstract

Aim To investigate the effect of dentine conditioning agents on growth factor liberation and settlement of Dental Pulp Progenitor Cells (DPSCs) on dentine surfaces. Methodology The agents used included ethylenediaminetetraacetic acid (EDTA; 10%, pH 7.2), phosphoric acid (37%, pH<1), citric acid (10%, pH 1.5) and polyacrylic acid (25%, pH 3.9). Human dentine slices were conditioned for exaggerated conditioning times of 5 and 10 minutes, so that the growth factor liberation reached quantifiable levels above the limit of detection of the laboratory methods employed. Transforming growth factor beta1 (TGF-β1) release and surface exposure were quantified by Enzyme-linked immunosorbent assay (ELISA) and immunogold labelling. Scanning electron microscopy (SEM) was used to assess the morphology of cells and coverage by DPSCs cultured on dentine surfaces for 8 days. Results After 5-minutes conditioning of dentine slices, citric acid was the most effective agent for growth factor release into the aqueous environment as measured by ELISA (Mann Whitney U with Bonferroni correction, P<0.01 compared with phosphoric and polyacrylic acid). As well as this, dentine slices treated with phosphoric acid for the same period, displayed significantly less TGF-β1 on the surface compared with the other agents used, as measured by immunogold labelling (MWU with Bonferroni correction, P<0.05). After 8 days, widespread coverage by DPSCs on dentine surfaces conditioned with citric acid and EDTA were evident under SEM. On dentine surfaces conditioned with phosphoric and polyacrylic acid respectively, less spread cells and inconsistent cell coverage were observed. In conclusion Based on the findings of this in vitro study, a desirable biological growth factor mediated effect may be gained when conditioning dentine by milder acidic or chelating agents such as citric acid and EDTA. The results must be interpreted in the context that the potential of the applied materials in inducing a desirable biological response in DPSCs, is only one consideration amongst other important ones in a clinical setting. However, it is crucial to look beyond the mere physical effects of materials and move towards biologically based treatment approaches as far as the restorative management of teeth with viable dental pulps are concerned.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Dentistry
Subjects:	R Medicine > RK Dentistry
Additional Information:	This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Publisher:	Wiley
ISSN:	0143-2885
Date of First Compliant Deposit:	8 June 2022
Date of Acceptance:	30 May 2022
Last Modified:	22 Mar 2024 02:08
URI:	https://orca.cardiff.ac.uk/id/eprint/150295

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