Generated randomly and selected functionally? The nature of enterovirus recombination

Alnaji, Fadi G., Bentley, Kirsten ORCID: https://orcid.org/0000-0002-6619-2098, Pearson, Ashley, Woodman, Andrew, Moore, Jonathan, Fox, Helen, Macadam, Andrew J. and Evans, David J. 2022. Generated randomly and selected functionally? The nature of enterovirus recombination. Viruses 14 (5) , 916. 10.3390/v14050916

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Abstract

Genetic recombination in RNA viruses is an important evolutionary mechanism. It contributes to population diversity, host/tissue adaptation, and compromises vaccine efficacy. Both the molecular mechanism and initial products of recombination are relatively poorly understood. We used an established poliovirus-based in vitro recombination assay to investigate the roles of sequence identity and RNA structure, implicated or inferred from an analysis of circulating recombinant viruses, in the process. In addition, we used next-generation sequencing to investigate the early products of recombination after cellular coinfection with different poliovirus serotypes. In independent studies, we find no evidence for a role for RNA identity or structure in determining recombination junctions location. Instead, genome function and fitness are of greater importance in determining the identity of recombinant progeny. These studies provide further insights into this important evolutionary mechanism and emphasize the critical nature of the selection process on a mixed virus population.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Additional Information:	This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Publisher:	MDPI
ISSN:	1999-4915
Date of First Compliant Deposit:	30 June 2022
Date of Acceptance:	26 April 2022
Last Modified:	18 May 2023 01:13
URI:	https://orca.cardiff.ac.uk/id/eprint/150920

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