Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies

Baxani, D. K., Jamieson, W. D. ORCID: https://orcid.org/0000-0001-8260-5211, Barrow, D. A. ORCID: https://orcid.org/0000-0003-2096-7262 and Castell, O. K. ORCID: https://orcid.org/0000-0002-6059-8062 2022. Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies. Soft Matter 18 , pp. 5089-5096. 10.1039/D1SM01111A

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Abstract

Whilst it is highly desirable to produce artificial lipid bilayer arrays allowing for systematic high-content screening of membrane conditions, it remains a challenge due to the combined requirements of scaled membrane production, simple measurement access, and independent control over individual bilayer experimental conditions. Here, droplet bilayers encapsulated within a hydrogel shell are output individually into multi-well plates for simple, arrayed quantitative measurements. The afforded experimental throughput is used to conduct a 2D concentration screen characterising the synergistic pore-forming peptides Magainin2 and PGLa. Maximal enhanced activity is revealed at equimolar peptide concentrations via a membrane dye leakage assay, a finding consistent with models proposed from NMR data. The versatility of the platform is demonstrated by performing in situ electrophysiology, revealing low conductance pore activity (∼15 to 20 pA with 4.5 pA sub-states). In conclusion, this array platform addresses the aforementioned challenges and provides new and flexible opportunities for high-throughput membrane studies. Furthermore, the ability to engineer droplet networks within each construct paves the way for “lab-in-a-capsule” approaches accommodating multiple assays per construct and allowing for communicative reaction pathways.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Publisher:	Royal Society of Chemistry
ISSN:	1744-683X
Funders:	EU H2020 ACDC G.A. n° 82406
Date of First Compliant Deposit:	6 July 2022
Date of Acceptance:	9 June 2022
Last Modified:	28 Apr 2024 17:54
URI:	https://orca.cardiff.ac.uk/id/eprint/151089

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