Immobilised teicoplanin does not demonstrate antimicrobial activity against Staphylococcus aureus

Britton, S., Lee, K., Azizova, L., Shaw, G., Nishio Ayre, W. ORCID: https://orcid.org/0000-0003-2405-1876 and Mansell, J. P. 2022. Immobilised teicoplanin does not demonstrate antimicrobial activity against Staphylococcus aureus. Scientific Reports 12 (1) , 16661. 10.1038/s41598-022-20310-8

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Abstract

Antibacterial bone biomaterial coatings appeal to orthopaedics, dentistry and veterinary medicine. Achieving the successful, stable conjugation of suitable compounds to biomaterial surfaces is a major challenge. A pragmatic starting point is to make use of existing, approved antibiotics which are known to remain functional in a stationary, immobilised state. This includes the macrocyclic glycopeptide, teicoplanin, following the discovery, in the 1990’s, that it could be used as a chiral selector in chromatographic enantiomeric separations. Importantly teicoplanin works at the level of the bacterial cell wall making it a potential candidate for biomaterial functionalisations. We initially sought to functionalise titanium (Ti) with polydopamine and use this platform to capture teicoplanin, however we were unable to avoid the natural affinity of the antibiotic to the oxide surface of the metal. Whilst the interaction between teicoplanin and Ti was robust, we found that phosphate resulted in antibiotic loss. Before contemplating the covalent attachment of teicoplanin to Ti we examined whether a commercial teicoplanin stationary phase could kill staphylococci. Whilst this commercially available material could bind N-Acetyl-L-Lys-D-Ala-D-Ala it was unable to kill bacteria. We therefore strongly discourage attempts at covalently immobilising teicoplanin and/or other glycopeptide antibiotics in the pursuit of novel antibacterial bone biomaterials.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Dentistry
Publisher:	Nature Research
ISSN:	2045-2322
Funders:	Versus Arthritis
Date of First Compliant Deposit:	10 October 2022
Date of Acceptance:	12 September 2022
Last Modified:	23 May 2023 14:32
URI:	https://orca.cardiff.ac.uk/id/eprint/153266

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