Death associated protein-3 (DAP3) and DAP3 binding cell death enhancer-1 (DELE1) in human colorectal cancer, and their impacts on clinical outcome and chemoresistance

Sui, Laijian, Zhang, Jianyuan, Zhao, Huishan, Ye, Lin ORCID: https://orcid.org/0000-0002-0303-2409, Martin, Tracey A. ORCID: https://orcid.org/0000-0003-2690-4908, Sanders, Andrew J., Ruge, Fiona, Jiang, A., Dou, Q. Ping, Hargest, Rachel ORCID: https://orcid.org/0000-0001-9830-3832, Song, Xicheng and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2022. Death associated protein-3 (DAP3) and DAP3 binding cell death enhancer-1 (DELE1) in human colorectal cancer, and their impacts on clinical outcome and chemoresistance. International Journal of Oncology 62 (1) , 7. 10.3892/ijo.2022.5455

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Abstract

Death associated protein‑3 (DAP3) was identified as a responsive protein to interferon‑gamma‑induced cell death which possibly exerts this regulation by interacting with DAP3 binding cell Death enhancer‑1 (DELE1), a newly discovered mitochondrial stress protein in response to cell stress signals. Whilst DAP3 has been shown to be aberrantly expressed in several cancer types (i.e. breast cancer), little is known about the relationship between DAP3 and DELE1 in cancers. The present study examined the expression levels of both DAP3 and DELE1 in clinical colorectal cancers (CRCs), as well as their implication on chemoresistance and mechanism behind the action. Firstly, transcript levels of both DAP3 and DELE1 were quantitatively assessed in a clinical cohort of CRC (n=94). Tumour tissues had significantly higher levels of DAP3, but not DELE1 compared with normal tissues. Levels of DAP3 and DELE1 had a significant association with patient's clinical outcomes and local recurrence. DAP3 and DELE1 significantly correlated in normal colorectal tissues but not in tumour tissues. Secondly, the protein levels of DAP3 and DELE1 were evaluated in both normal and tumour colon tissues which showed that both proteins were highly aberrant in CRC tissues. In addition, both DAP3 and DELE1 at transcript and protein levels were identified as prognostic factors for patient's clinical outcomes. Furthermore, in in vitro assays, knocking down DAP3 or DELE1, and in particular both DAP3 and DELE1 together rendered the CRC cells more sensitive to chemotherapy drugs, consistent with clinical findings of the TCGA‑COAD datasets. The acquisition of drug sensitivity following the genetic knockdown was independent of the mitochondrial metabolism, as neither DAP3 knockdown nor DELE1 knockdown showed a significant change. In summary, DAP3 and DELE1 are highly aberrant in CRCs, and both molecules are prognostic factors for patient's clinical outcomes and local recurrence and are indicators for chemoresistance.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Medicine
Publisher:	Spandidos Publications
ISSN:	1791-2423
Funders:	Cardiff China Medical Scholarship, Taishan Scholars Project (grant no. ts20190991) and RealCan Fellowship
Date of First Compliant Deposit:	16 November 2022
Date of Acceptance:	14 October 2022
Last Modified:	10 Jun 2023 18:39
URI:	https://orca.cardiff.ac.uk/id/eprint/153822

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