Development, characterization and antimicrobial activity of multilayer silica nanoparticles with chlorhexidine incorporated into dental composites

Larissa, Pavanello, Gambrill, Benjamin, de Carvalho, Rafaela Durrer Parolina, Picolo, Mayara Zagui Dal, Cavalli, Vanessa, Boaro, Letícia Cristina Cidreira, Prokopovich, Polina ORCID: https://orcid.org/0000-0002-5700-9570 and Cogo-Müller, Karina 2023. Development, characterization and antimicrobial activity of multilayer silica nanoparticles with chlorhexidine incorporated into dental composites. Dental Materials 39 (5) , pp. 469-477. 10.1016/j.dental.2023.03.005

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Abstract

Objective In this study a dentistry nanocomposite with prolonged antibacterial activity using silica nanoparticles (SNPs) loaded with chlorhexidine (CHX) was developed. Methods SNPs were coated with the Layer-by-Layer technique. Dental composites were prepared with organic matrix of BisGMA/TEGDMA and SNPs with or without CHX (0, 10, 20 or 30% w/w). The physicochemical properties of the developed material were evaluated and agar diffusion method was used to test the antibacterial. In addition, the biofilm inhibitory activity of the composites was evaluated against S. mutans. Results SNPs were rounded with diameters about 50 nm, the organic load increased with increasing deposited layers. Material samples with SNPs loaded with CHX (CHX-SNPs) showed the highest values of post-gel volumetric shrinkage, that ranged from 0.3% to 0.81%. Samples containing CHX-SNPs 30% w/w showed the highest values of flexural strength and modulus of elasticity. Only samples containing SNPs-CHX showed growth inhibition against S. mutans, S. mitis and S. gordonii in a concentration-dependent manner. The composites with CHX-SNPs reduced the biofilm formation of S. mutans biofilm at 24 h and 72 h. Significance The nanoparticle studied acted as fillers and did not interfere with the evaluated physicochemical properties while providing antimicrobial activity against streptococci. Therefore, this initial study is a step forward to the synthesis of experimental composites with improved performance using CHX-SNPs.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Additional Information:	License information from Publisher: LICENSE 1: URL: http://creativecommons.org/licenses/by/4.0/, Start Date: 2023-03-04
Publisher:	Elsevier
ISSN:	0109-5641
Date of First Compliant Deposit:	20 March 2023
Date of Acceptance:	3 March 2023
Last Modified:	04 May 2023 08:25
URI:	https://orca.cardiff.ac.uk/id/eprint/157814

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