Shu proteins promote the formation of homologous recombination intermediates that are processed by sgs1-rmi1-top3

Mankouri, Hocine W., Ngo, Greg and Hickson, Ian D. 2007. Shu proteins promote the formation of homologous recombination intermediates that are processed by sgs1-rmi1-top3. Molecular Biology of the Cell 18 (10) , pp. 3711-4200. 10.1091/mbc.e07-05-0490

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Abstract

CSM2, PSY3, SHU1, and SHU2 (collectively referred to as the SHU genes) were identified in Saccharomyces cerevisiae as four genes in the same epistasis group that suppress various sgs1 and top3 mutant phenotypes when mutated. Although the SHU genes have been implicated in homologous recombination repair (HRR), their precise role(s) within this pathway remains poorly understood. Here, we have identified a specific role for the Shu proteins in a Rad51/Rad54-dependent HRR pathway(s) to repair MMS-induced lesions during S-phase. We show that, although mutation of RAD51 or RAD54 prevented the formation of MMS-induced HRR intermediates (X-molecules) arising during replication in sgs1 cells, mutation of SHU genes attenuated the level of these structures. Similar findings were also observed in shu1 cells in which Rmi1 or Top3 function was impaired. We propose a model in which the Shu proteins act in HRR to promote the formation of HRR intermediates that are processed by the Sgs1-Rmi1-Top3 complex.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Medicine
Publisher:	American Society for Cell Biology
ISSN:	1939-4586
Date of First Compliant Deposit:	11 April 2023
Date of Acceptance:	18 July 2007
Last Modified:	19 May 2023 19:22
URI:	https://orca.cardiff.ac.uk/id/eprint/158410

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