A novel whole-body thermal stress test for monitoring cardiovascular responses in guinea pigs

Sixtus, Ryan P. ORCID: https://orcid.org/0000-0002-2041-8928, Berry, Mary J., Gray, Clint L. and Dyson, Rebecca M. 2023. A novel whole-body thermal stress test for monitoring cardiovascular responses in guinea pigs. Journal of Thermal Biology 113 , 103500. 10.1016/j.jtherbio.2023.103500

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Abstract

Cardiovascular disease is a leading cause of morbidity and mortality worldwide. Stress tests are frequently employed to expose early signs of cardiovascular dysfunction or disease and can be employed, for example, in the context of preterm birth. We aimed to establish a safe and effective thermal stress test to examine cardiovascular function. Guinea pigs were anaesthetized using a 0.8% isoflurane, 70% N2O mix. ECG, non-invasive blood pressure, laser Doppler flowmetry, respiratory rate, and an array of skin and rectal thermistors were applied. A physiologically relevant heating and a cooling thermal stress test was developed. Upper and lower thermal limits for core body temperature were set at 41.5 OC and 34 OC, for the safe recovery of animals. This protocol therefore presents a viable thermal stress test for use in guinea pig models of health and disease that facilitates exploration of whole-system cardiovascular function.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Biosciences
Publisher:	Elsevier
ISSN:	0306-4565
Date of First Compliant Deposit:	6 April 2023
Date of Acceptance:	4 February 2023
Last Modified:	02 May 2023 11:18
URI:	https://orca.cardiff.ac.uk/id/eprint/158479

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